NOTICE: You are viewing a page of the openwetware wiki. Our "dewikify" feature makes a wiki page appear as a normal web page. On September 22nd 2017, this feature will GO AWAY and this URL will redirect to the source URL on our wiki. We're sorry for the inconvenience.
Lauffenburger Lab

Image:wikiburger_top.jpg

Home        People        Research        Publications        Links        Internal       

Megan Palmer (BE doctoral)

Integration of T Cell Receptor and Interleukin 7 Cytokine Signaling for Network Control of Naïve CD8+ T Cell Fate

The transfer of naïve T cells into lymphopenic hosts results in their proliferation and differentiation to a memory phenotype. Lymphopenia-induced proliferation is critical to reconstitution of the immune system following T cell depletion by viral immunodeficiency or chemotherapy, and provides insight into the mechanisms of T cell maintenance. T cell survival and proliferation is dependent upon T cell receptor (TCR) stimulation by self-peptide/major histocompatibility complex (spMHC) in combination with cytokine signaling by interleukin-7 (IL-7) through the IL-7 receptor (IL-7R). IL-7R and TCR stimuli are thought to act synergistically in their regulation of T cell expansion. However, the mechanisms of signaling cross-talk are not known.

To elucidate how TCR and IL-7R signals direct cellular responses, we are developing an in vitro system to enable controlled TCR and IL-7R stimulation. Dynamic signaling activities across common downstream pathways will be simultaneously measured alongside phenotypic responses to characterize network behavior governing distinct T cell fates. Statistical and/or probabilistic modeling approaches will be used to derive from these portraits of signaling behavior predicted mechanisms of regulation and synergy in the TCR-IL7 network.